Prevalence and molecular characterization of extended spectrum beta-lactamase producing uropathogenic Escherichia coli in a tertiary health care facility in Anambra State, Nigeria

Background: The prevalence of ESBLs-producing Escherichia coli has recently increased worldwide due to the expression ESBL genes which had led high rate multidrug resistance antibiotics. Aim: To determine antimicrobial susceptibility patterns E. isolates and evaluating carriage these at phenotypic genotypic levels. Methods: One hundred two clinical were recovered from UTI suspects analyzed for production levels using Modified Double Disc Synergy Test Polymerase Chain Reaction respectively. Results: Of 102 isolates, 100(98.04%) associated with MDR phenotypes. showed variable all antibiotics used in study. rates 99.0%, 97.1%, 88.2%, 82.4%, 81.4%, 65.7%, 54.9%, 46.1%, 23.5% ampicillin, trimethoprim-sulfamethoxazole, ciprofloxacin, ceftriaxone, ceftazidime, amoxicillin-clavulanate, gentamycin, cefoxitin, nitrofurantoin imipenem, was 74.5%. Based on PCR results, randomly selected 20 ESBL-positive possessed one or more ESBLs genes. CTX-M type predominant (100%), while those TEM SHV-types 85% 80% Four genotype detected (CTX-M, TEM+CTX-M, SHV+CTX-M SHV+TEM+CTX-M). SHV+TEM+CTX-M, (70%), followed by +CTX-M combination (15%). occurrences genotypes, 5% 10% Conclusion: This study found a Phenotypic (74.5%) among resistance, as (100%) SHV+TEM+CTX-M (70%).